POLR1A Antibody PA5-65577 from Thermo Fisher Scientific

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Western blot was performed using Anti-POLR1A Polyclonal Antibody (Product # PA5-65577) and a 195 kDa band corresponding to POLR1A was observed along with uncharacterized bands (*) across cell lines tested. Whole cell Extracts (1% SDS) (30 µg lysate) of K-562 (Lane 1), Caco-2 (Lane 2), A549 (Lane 3), HeLa (Lane 4), NTERA-2 (Lane 5), MCF-7 (Lane 6) and RAW 264.7 (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by wet transfer XCell SureLock™ Mini-Cell and XCell II™ Blot Module (Product # EI0002). The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4,000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
ICC/IF

Immunofluorescence analysis of POLR1A was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with POLR1A Polyclonal Antibody (Product # PA5-65577) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415). Panel d represents the merged image showing Nucleolar localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

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Thermo Fisher Scientific for
POLR1A Antibody

Thermo Fisher Scientific's POLR1A Antibody is a Rabbit Polyclonal antibody. This antibody has been shown to work in applications such as: Immunocytochemistry. The POLR1A Antibody was generated using POLR1A as the antigen and it reacts with Homo Sapiens, and Human.

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POLR1A Antibody from Thermo Fisher Scientific

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POLR1A Antibody from Thermo Fisher Scientific

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